Enantiomeric separation of thiourea derivatives of naringenin on amylose and cellulose polymeric chromatographic chiral columns.

Due to a great demand for amylose and cellulose polymeric chromatographic chiral columns, the enantiomeric separation of thiourea derivatives of naringenin was achieved on the different amylose (Chiralpak-IB) and cellulose chiral (Chiralcel-OJ and Chiralcel-OD-3R) columns with varied chromatographic conditions. The isocratic mobile phases used were ethanol and methanol, where ethanol/hexane and methanol/hexane were used as gradient mode and were prepared in volume/volume relation. The separation and resolution factors for all the enantiomers were in the range of 1.25 to 3.47 and 0.48 to 1.75, respectively. The enantiomeric resolution was obtained within 12 min making fast separation. The docking studies confirmed the chiral recognition mechanisms with binding affinities in the range of -4.7 to -5.7 kcal/mol. The reported compounds have good anticoagulant activities and may be used as anticoagulants in the future. Besides, chiral separation is fast and is useful for enantiomeric separation in any laboratory in the world.

Chitosan-grafted salicylaldehyde/algae composite for methyl violet dye removal: adsorption modeling and optimization.

In this study, a hydrothermal approach was employed to graft chitosan (Chit)/algae (ALG) with salicylaldehyde (SA), resulting in the synthesis of a biocomposite named salicylaldehyde-based chitosan Schiff base/algae (Chit-SA/ALG). The main objective of this biocomposite was to effectively remove methyl violet (MV), an organic dye, from aqueous solutions. The adsorption performance of Chit-SA/ALG toward MV was investigated in detail, considering the effects of three factors: (A) Chit-SA/ALG dose (ranging from 0.02 to 0.1 g/100 mL), (B) pH (ranging from 4 to 10), and (C) time (ranging from 10 to 120 min). The Box-Behnken design (BBD) was utilized for experimental design and analysis. The experimental results exhibited a good fit with both the pseudo-second-order kinetic model and the Freundlich isotherm, suggesting their suitability for describing the MV adsorption process on Chit-SA/ALG. The maximum adsorption capacity of Chit-SA/ALG, as calculated by the Langmuir model, was found to be 115.6 mg/g. The remarkable adsorption of MV onto Chit-SA/ALG can be primarily attributed to the electrostatic forces between Chit-SA/ALG and MV as well as the involvement of various interactions such as n-π, π-π, and H-bond interactions. This research demonstrates that Chit-SA/ALG exhibits promising potential as a highly efficient adsorbent for the removal of organic dyes from water systems.

The novelty of this work comes from introducing a new bio-organic based composite adsorbent of chitosan (Chit) biopolymer and algae (ALG) biomass. Moreover, the functionality and chemical stability of Chit­ALG composite was further developed by grafting process with salicylaldehyde (SA) using hydrothermal process. The incorporation of ALG biomass into polymeric matrix of Chit and grafting process with SA makes Chit a unique hybrid adsorbent toward cationic dye (methyl violet dye).

Amine-Decorated Methacrylic Acid-based Inverse Vulcanized Polysulfide for Effective Mercury Removal from Wastewater.

Mercury [Hg(II)] contamination is an indefatigable global hazard that causes severe permanent damage to human health. Extensive research has been carried out to produce mercury adsorbents; however, they still face certain challenges, limiting their upscaling. Herein, we report the synthesis of a novel amine-impregnated inverse vulcanized copolymer for effective mercury removal. Poly(S-MA) was prepared using sulfur and methacrylic acid employing the inverse vulcanization method, followed by functionalization. The polyethylenimine (PEI) was impregnated on poly(S-MA) to increase the adsorption active sites. The adsorbent was then characterized byusing Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). FTIR spectroscopy confirmed the formation of the copolymer, and successful impregnation of PEI and SEM revealed the composite porous morphology of the copolymer. Amine-impregnated copolymer [amine@poly(S-MA)] outperformed poly(S-MA) in mercury as it showed 20% superior performance with 44.7 mg/g of mercury adsorption capacity. The adsorption data best fit the pseudo-second-order, indicating that chemisorption is the most effective mechanism, in this case, indicating the involvement of NH2 in mercury removal. The adsorption is mainly a monolayer on a homogeneous surface as indicated by the 0.76 value of Redlich-Peterson exponent (g), which describes the adsorption nature advent from the R2 value of 0.99.

Role of organic and inorganic amendments on physiological attributes of germinating pea seedlings under arsenic stress.

There are scarce data regarding the effects of soil amendments on biophysicochemical responses of plants at the early stages of growth/germination. This study critically compares the effects of ethylene-diamine-tetra-acetic-acid (EDTA) and calcium (Ca) on biophysicochemical responses of germinating pea seedlings under varied arsenic levels (As, 25, 125, 250 µM). Arsenic alone enhanced hydrogen peroxide (H2O2) level in pea roots (176%) and shoot (89%), which significantly reduced seed germination percentage, pigment contents, and growth parameters. Presence of EDTA and Ca in growth culture minimized the toxic effects of As on pea seedlings, EDTA being more pertinent than Ca. Both the amendments decreased H2O2 levels in pea tissues (16% in shoot and 13% in roots by EDTA, and 7% by Ca in shoot), and maintained seed germination, pigment contents, and growth parameters of peas close to those of the control treatment. The effects of all As-treatments were more pronounced in the pea roots than in the shoot. The presence of organic and inorganic amendments can play a useful role in alleviating As toxicity at the early stages of pea growth. The scarcity of data demands comparing plant biophysicochemical responses at different stages of plant growth (germinating vs mature) in future studies.

Till date, abundant research has focused on plant biophysicochemical responses to different types of pollutants. However, the majority of these studies dealt with pollutant exposure to mature plants (generally after a vegetative growth period of 1­2 weeks). Despite significant research, there are still limited data regarding the biophysicochemical responses of plants at their early stages of germination and growth. In fact, stresses at germination or at an early stage of growth can be highly fatal and may significantly affect the ultimate plant growth and potential to remediate the contaminated sites. Therefore, the current study deals with the exposure of germinating pea seedlings to arsenic (As) stress under varied amendments. This experimental plan helped to understand the initial biophysicochemical changes induced in pea plants under As stress.

Forensic analysis of mosquito blood meal digestion process and the impact of heroin opiate: determination of the post-feeding interval as a PMI estimation.

Females of some mosquito species are anthropophilic, as they feed on human blood to support egg production and, hence, are forensically valuable if found at a crime scene. The present study investigated the blood meal digestion process in Culex pipiens L. both with and without heroin and proposed a method for estimating the post-feeding interval (PFI). Mosquitoes were fed on a control mouse, a heroin-injected mouse, or in vitro heroin-treated mouse blood. The blood meal digestion was then investigated at different hours post-feeding. Data showed that the blood meal size ingested by control mosquitoes was 0.681 ±â€…0.04 mg/mosquito and was completely digested within 45 h post-feeding. An estimation of the PFI was proposed in terms of the rate of hemoglobin (Hb) digestion. The blood meal size of the mosquitoes fed on the in vitro heroin-treated blood and the heroin-injected mouse was 0.96 ±â€…0.06 and 0.79 ±â€…0.01 mg/mosquito and was completely digested within 50 and 55 h post-feeding, respectively. The digestion of Hb started similarly in all experimental mosquitoes until 10 h post-feeding, after which it significantly decreased in heroin-treated blood meals compared with the control ones. This may suggest that heroin impacted the digestion process, as it took an extra 5-10 h to complete. These findings could be valuable in the forensic context since an estimation of PFI is proposed as a potential estimation of the postmortem interval (PMI). However, care should be taken as heroin in the host blood has significantly impacted the overall digestion process and, hence, may bias the PFI/PMI estimation.

Biomagnetic chitosan-ethylene glycol diglycidyl ether/organo-nanoclay nanocomposite for azo dye removal: A statistical modeling by response surface methodology.

Herein, a quadruple biomagnetic nanocomposite of cross-linked chitosan-ethylene glycol diglycidyl ether/organo-nanoclay (MCH-EGDE/ORNC) was designed for the uptake of remazol brilliant blue R (RBBR) dye from aqueous environment. The adsorption process was systematically improved via the Box-Behnken design (BBD) to determine the influence of key uptake parameters, including MCH-EGDE/ORNC dosage, pH, and time, on the RBBR removal. The highest RBBR removal of 87.5 % was achieved at the following conditions: MCH-EGDE/ORNC dosage: 0.1 g/100 mL; pH: 4.0; contact time: 25 min. The findings of the kinetics and equilibrium studies revealed an excellent fit to the pseudo-second order and the Freundlich models, respectively. The adsorption capacity of the MCH-EGDE/ORNC for RBBR was found to be 168.4 mg/g, showcasing its remarkable adsorption capability. The present work highlights the potential of MCH-EGDE/ORNC biomaterial as an advanced adsorbent and lays the foundation for future applications in water purification and environmental remediation.

Organically modified montmorillonite composited with magnetic glyoxal-chitosan Schiff base for reactive blue 19 dye removal: Process optimization and adsorptive mechanism.

In this study, a new biocomposite magnetic adsorbent (magnetic glyoxal-chitosan Schiff base/organically modified montmorillonite (MCTS-GOX/OMMT)) was synthesized and employed for the adsorption of reactive blue 19 dye (RB19) from aqueous environment. The physicochemical properties of the MCTS-GOX/OMMT were confirmed by using various characterization techniques such as BET, XRD, FTIR, SEM-EDX, VSM, and pHpzc. The adsorption key variables were statistically optimized via Box-Behnken design (BBD) And accordingly the best operational conditions to achieve maximum RB19 removal were recorded at MCTS-GOX/OMMT dosage = 0.1 g/0.1 L, solution pH = 4, and working temperature = 25 °C. The adsorption process for RB19 appeared to follow the pseudo-second-order kinetic and the Langmuir isotherm models, according to the findings of the adsorption kinetics and equilibrium investigations. The maximum adsorption capacity of the MCTS-GOX/OMMT towards RB19 was 122.3 mg/g, demonstrating its preferable adsorption capability. The successful development of this novel magnetic bioadsorbent with excellent adsorption ability towards organic dyes and efficient separation ability opens possibilities for its practical application in wastewater treatment and dye removal processes.

Bisphenol-A-diglycidyl ether modified chitosan/nano-SiO2 via hydrothermal process: A statistical modeling and adsorption mechanism for reactive orange 16 dye removal.

In this study, chitosan/nano SiO2 (CTS/NS) was chemically modified with bisphenol A diglycidyl ether (BADGE) cross-linker-assisted hydrothermal process to create an effective adsorbent, CTS-BADGE/NS, for the removal of reactive orange 16 (RO16) dye from aquatic systems. Box-Behnken design (BBD) was used to optimize the adsorption process by varying the adsorbent dose (0.02-0.1 g/100 mL), pH (4-10), and time (20-360 min). The adsorption isotherm results indicated that the Langmuir model fits the experimental data well, suggesting that the adsorption process involves a monolayer formation of RO16 on the surface of CTS-BADGE/NS. The kinetic modeling of RO16 adsorption by CTS-BADGE/NS demonstrated that the pseudo-first-order model fits the adsorption data. CTS-BADGE/NS achieved an adsorption capacity of 97.8 mg/g for RO16 dye at optimum desirability functions of dosage 0.099 g/100 mL, solution pH of 4.44, and temperature of 25 °C. Overall, the π-π electron donor-acceptor system significantly improved the adsorption performance of the CTS-BADGE/NS. The results of the regeneration investigation demonstrate that the CTS-BADGE/NS exhibits effective adsorption of RO16, even after undergoing five consecutive cycles. The results of this study suggest that the developed CTS-BADGE/NS composite can be a promising adsorbent for water purification applications.

Food-grade algae modified Schiff base-chitosan benzaldehyde composite for cationic methyl violet 2B dye removal: RSM statistical parametric optimization.

This work aims to apply the use of food-grade algae (FGA) composited with chitosan-benzaldehyde Schiff base biopolymer (CHA-BD) as a new adsorbent (CHA-BA/FGA) for methyl violet 2B (MV 2B) dye removal from aqueous solutions. The effect of three processing variables, including CHA-BA/FGA dosage (0.02-0.1 g/100 mL), pH solution (4-10), and contact duration (10-120 min) on the removal of MV 2B was investigated using the Box-Behnken design (BBD) model. Kinetic and equilibrium dye adsorption profiles reveal that the uptake of MV 2B dye by CHA-BA/FGA is described by the pseudo-second kinetics and the Langmuir models. The thermodynamics of the adsorption process (ΔG°, ΔH°, and ΔS°) reveal spontaneous and favorable adsorption parameters of MV 2B dye onto the CHA-BA/FGA biocomposite at ambient conditions. The CHA-BA/FGA exhibited the maximum ability to absorb MV 2B of 126.51 mg/g (operating conditions: CHA-BA/FGA dose = 0.09 g/100 mL, solution pH = 8.68, and temperature = 25 °C). Various interactions, including H-bonding, electrostatic forces, π-π stacking, and n-π stacking provide an account of the hypothesized mechanism of MV 2B adsorption onto the surface of CHA-BA/FGA. This research reveals that CHA-BA/FGA with its unique biocomposite structure and favorable adsorption properties can be used to remove harmful cationic dyes from wastewater.

The first novel aspect of this research work comes from the utilization of food-grade algae which contains various types of negative functional groups hydroxyl, carboxyl, and phosphate to modify a cationic biopolymer (Chitosan) to improve its adsorptive property toward removal of a cationic dye such as methyl violet 2B. The second novel aspect of this research work is to use the hydrothermal process to assist the grafting of an aromatic ring of benzaldehyde into the polymer matrix of the chitosan-food grade algae composite via a Schiff base linkage to improve its chemical stability and functionality.

Optimization and mechanistic approach for removal of crystal violet and methylene blue dyes via activated carbon from pyrolyzed-ZnCl2 bamboo waste.

In this study, bamboo waste (BW) was subjected to pyrolysis-assisted ZnCl2 activation to produce mesoporous activated carbon (BW-AC), which was then evaluated for its ability to remove cationic dyes, specifically methylene blue (MB) and crystal violet (CV), from aqueous environments. The properties of BW-AC were characterized using various techniques, including potentiometric-based point of zero charge (pHpzc), scanning electron microscopy with energy dispersive X-rays (SEM-EDX), X-ray diffraction (XRD), gas adsorption with Brunauer-Emmett-Teller (BET) analysis, infrared (IR) spectroscopy. To optimize the adsorption characteristics (BW-AC dosage, pH, and contact time) of PBW, a Box-Behnken design (BBD) was employed. The BW-AC dose of 0.05 g, solution pH of 10, and time of 8 min are identified as optimal operational conditions for achieving maximum CV (89.8%) and MB (96.3%) adsorption according to the BBD model. The dye removal kinetics for CV and MB are described by the pseudo-second-order model. The dye adsorption isotherms revealed that adsorption of CV and MB onto BW-AC follow the Freundlich model. The maximum dye adsorption capacities (qmax) of BW-AC for CV (530 mg/g) and MB (520 mg/g) are favorable, along with the thermodynamics of the adsorption process, which is characterized as endothermic and spontaneous. The adsorption mechanism of CV and MB dyes by BW-AC was attributed to multiple contributions: hydrogen bonding, electrostatic forces, π-π attraction, and pore filling. The findings of this study highlight the potential of BW-AC as an effective adsorbent in wastewater treatment applications, contributing to the overall goal of mitigating the environmental impact of cationic dyes and ensuring the quality of water resources.

The novelty of this research work comes from the conversion of the bamboo waste (BW) into mesoporous activated carbon (BW-AC) via pyrolysis-assisted ZnCl₂ activation for the removal of cationic dyes such as methylene blue (MB) and crystal violet (CV) from aqueous media. The effectiveness of the obtained activated carbon was tested toward removal of two structurally different cationic dyes (CV and MB), where a statistical optimization employing a response surface methodology with Box-Behnken design was applied to optimize dye removal. In addition to determination of the working parameters for dye removal, the adsorption kinetics and thermodynamic parameters for the adsorption process were determined to provide molecular-level insight.

High surface area activated carbon from a pineapple (ananas comosus) crown via microwave-ZnCl2 activation for crystal violet and methylene blue dye removal: adsorption optimization and mechanism.

In this investigation, microwave irradiation assisted by ZnCl2 was used to transform pineapple crown (PN) waste into mesoporous activated carbon (PNAC). Complementary techniques were employed to examine the physicochemical characteristics of PNAC, including BET, FTIR, SEM-EDX, XRD, and pH at the point-of-zero-charge (pHpzc). PNAC is mesoporous adsorbent with a surface area of 1070 m2/g. The statistical optimization for the adsorption process of two model cationic dyes (methylene blue: MB and, crystal violet: CV) was conducted using the response surface methodology-Box-Behnken design (RSM-BBD). The parameters include solution pH (4-10), contact time (2-12) min, and PNAC dosage (0.02-0.1 g/100 mL). The Freundlich and Langmuir models adequately described the dye adsorption isotherm results for the MB and CV systems, whereas the pseudo-second order kinetic model accounted for the time dependent adsorption results. The maximum adsorption capacity (qmax) for PNAC with the two tested dyes are listed: 263.9 mg/g for CV and 274.8 mg/g for MB. The unique adsorption mechanism of MB and CV dyes by PNAC implicates multiple contributions to the adsorption process such as pore filling, electrostatic forces, H-bonding, and π-π interactions. This study illustrates the possibility of transforming PN into activated carbon (PNAC) with the potential to remove two cationic dyes from aqueous media.

The novelty of this research work stems from the conversion of pineapple (Ananas comosus) crown wastes with no monetary value into an efficient activated carbon adsorbent with relatively high surface area. Furthermore, a fast and convenient microwave assisted ZnCl₂ activation method was applied for producing the activated carbon (AC). The effectiveness of the produced AC was tested for the removal of two different cationic dyes: crystal violet (CV) and methylene blue (MB). A statistical optimization that employs a response surface methodology with the Box-Behnken design was employed to optimize the adsorption variables for the optimal dye removal. Moreover, the dye adsorption kinetics and thermodynamics, equilibrium isotherms, and the details of the adsorption process were reported herein.

Enhancing cationic dye removal via biocomposite formation between chitosan and food grade algae: Optimization of algae loading and adsorption parameters.

Herein, a natural material including chitosan (CTS) and algae (food-grade algae, FGA) was exploited to attain a bio-adsorbent (CTS/FGA) for enhanced methyl violet 2B dye removal. A study of the FGA loading into CTS matrix showed that the best mixing ratio between CTS and FGA to be used for the MV 2B removal was 50 %:50 % (CTS/FGA; 50:50 w/w). The present study employed the Box-Behnken design (RSM-BBD) to investigate the impact of three processing factors, namely CTS/FGA-(50:50) dose (0.02-0.1 g/100 mL), pH of solution (4-10), and contact time (5-15 min) on the decolorization rate of MV 2B dye. The results obtained from the equilibrium and kinetic experiments indicate that the adsorption of MV 2B dye on CTS/FGA-(50:50) follows the Langmuir and pseudo-second-order models, respectively. The CTS/FGA exhibits an adsorption capacity of 179.8 mg/g. The characterization of CTS/FGA-(50:50) involves the proposed mechanism of MV 2B adsorption, which primarily encompasses various interactions such as electrostatic forces, n-π stacking, and H-bonding. The present study demonstrates that CTS/FGA-(50:50) synthesized material exhibits a distinctive structure and excellent adsorption properties, thereby providing a viable option for the elimination of toxic cationic dyes from polluted water.

Ti3C2-MXene/NiO Nanocomposites-Decorated CsPbI3 Perovskite Active Materials under UV-Light Irradiation for the Enhancement of Crystal-Violet Dye Photodegradation.

Ti3C2-MXene material, known for its strong electronic conductivity and optical properties, has emerged as a promising alternative to noble metals as a cocatalyst for the development of efficient photocatalysts used in environmental cleanup. In this study, we investigated the photodegradation of crystal-violet (CV) dye when exposed to UV light using a newly developed photocatalyst known as Ti3C2-MXene/NiO nanocomposite-decorated CsPbI3 perovskite, which was synthesized through a hydrothermal method. Our research investigation into the structural, morphological, and optical characteristics of the Ti3C2-MXene/NiO/CsPbI3 composite using techniques such as FTIR, XRD, TEM, SEM-EDS mapping, XPS, UV-Vis, and PL spectroscopy. The photocatalytic efficacy of the Ti3C2-MXene/NiO/CsPbI3 composite was assessed by evaluating its ability to degrade CV dye in an aqueous solution under UV-light irradiation. Remarkably, the Ti3C2-MXene/NiO/CsPbI3 composite displayed a significant improvement in both the degradation rate and stability of CV dye when compared to the Ti3C2-MXene/NiO nanocomposite and CsPbI3 perovskite materials. Furthermore, the UV-visible absorption spectrum of the Ti3C2-MXene/NiO/CsPbI3 composite demonstrated a reduced band gap of 2.41 eV, which is lower than that of Ti3C2-MXene/NiO (3.10 eV) and Ti3C2-MXene (1.60 eV). In practical terms, the Ti3C2-MXene/NiO/CsPbI3 composite achieved an impressive 92.8% degradation of CV dye within 90 min of UV light exposure. We also confirmed the significant role of photogenerated holes and radicals in the CV dye removal process through radical scavenger trapping experiments. Based on our findings, we proposed a plausible photocatalytic mechanism for the Ti3C2-MXene/NiO/CsPbI3 composite. This research may open up new avenues for the development of cost-effective and high-performance MXene-based perovskite photocatalysts, utilizing abundant and sustainable materials for environmental remediation.

Mesoporous activated carbon derived from fruit by-product by pyrolysis induced chemical activation: optimization and mechanism for fuchsin basic dye removal.

In this study, pineapple crown (PC) feedstock residues were utilized as a potential precursor toward producing activated carbon (PCAC) via pyrolysis induced with ZnCl2 activation. The PCAC has a surface area (457.8 m2/g) and a mesoporous structure with an average pore diameter of 3.35 nm, according to the Brunauer-Emmett-Teller estimate. The removal of cationic dye (Fuchsin basic; FB) was used for investigating the adsorption parameters of PCAC. The optimization of significant adsorption variables (A: PCAC dose (0.02-0.1 g/100 mL); B: pH (4-10); C: time (10-90); and D: initial FB concentration (10-50 mg/L) was conducted using the Box-Behnken design (BBD). The pseudo-second-order (PSO) model characterized the dye adsorption kinetic profile, whereas the Freundlich model reflected the equilibrium adsorption profile. The maximum adsorption capacity (qmax) of PCAC for FB dye was determined to be 171.5 mg/g. Numerous factors contribute to the FB dye adsorption mechanism onto the surface of PCAC, which include electrostatic attraction, H-bonding, pore diffusion, and π-π stacking. This study illustrates the utilization of PC biomass feedstock for the fabrication of PCAC and its successful application in wastewater remediation.

Response surface methodology for optimizing methylene blue dye removal by mesoporous activated carbon derived from renewable woody Bambusoideae waste.

In this study, the focus was on utilizing tropical plant biomass waste, specifically bamboo (BB), as a sustainable precursor for the production of activated carbon (BBAC) via pyrolysis-induced K2CO3 activation. The potential application of BBAC as an effective adsorbent for the removal of methylene blue (MB) dye from aqueous solutions was investigated. Response surface methodology (RSM) was employed to evaluate key adsorption characteristics, which included BBAC dosage (A: 0.02-0.08 g/L), pH (B: 4-10), and time (C: 2-8 min). The adsorption isotherm analysis revealed that the adsorption of MB followed the Freundlich model. Moreover, the kinetic data were well-described by the pseudo-second-order model, suggesting the role of a chemisorption process. The BBAC demonstrated a notable MB adsorption capacity of 195.8 mg/g, highlighting its effectiveness as an adsorbent. Multiple mechanisms were identified as controlling factors in MB adsorption by BBAC, including electrostatic forces, π-π stacking, and H-bonding interactions. The findings of this study indicate that BBAC derived from bamboo has the potential to be a promising adsorbent for the treatment of wastewater containing organic dyes. The employment of sustainable precursors like bamboo for activated carbon production contributes to environmentally friendly waste management practices and offers a solution for the remediation of dye-contaminated wastewater.

This works introduces a renewable and woody Bambusoideae waste as promising and low-cost precursor for producing mesoporous activated carbon via microwave assisted K₂CO₃ activation. The effectiveness of the prepared activated carbon was tested toward removal of a toxic cationic dye, namely; methylene blue from aqueous environment.

Facile extraction of berberine from different plants, separation, and identification by thin-layer chromatography, high-performance liquid chromatography, and biological evaluation against Leishmaniosis.

The extraction of berberine was carried out from Berberis vulgaris, Berberis aquifolium, and Hydrastis canadensis plants using ethanol and water (70:30, v/v). The extracted berberine was characterized by ultraviolet-visible and Fourier-transform infrared spectroscopy. The purity of berberine was ascertained by thin-layer chromatography using n-propanol-formic acid-water (95:1:4) and (90:1:9) solvents. hRf values were in the range of 44-49 with compact spots (diameter 0.2-0.4 cm). HPLC was carried out using ammonium acetate buffer and acetonitrile in gradient mode with Zodiac (4.6 × 150 mm, 3 µm) column. The flow rate was 1.0 mL/min and detection was at 220 nm. The values of separation and resolution factors of the standard and the extracted berberine were in the range of 1.13-1.16 and 1.40-1.71, respectively. A comparison has shown that both thin-layer chromatography and high-performance liquid chromatography (HPLC) methods found applications in different situations and requirements. The extracted berberine samples were used to treat Leishmaniosis and the results showed better activity of berberine in comparison to the standard drug Amphotericin B. Briefly, the reported research is a novel and may be used to extract berberine from plants, separation and identification of berberine by thin layer chromatography and HPLC and to treat Leishmaniosis.

Physicochemical fabrication of chitosan and algae with crosslinking glyoxal for cationic dye removal: Insight into optimization, kinetics, isotherms, and adsorption mechanism.

Herein, a highly efficient and sustainable adsorbent of cross-linked chitosan-glyoxal/algae biocomposite (CHT-GLX/ALG) adsorbent was developed through an innovative hydrothermal cross-linking method. The CHT-GLX/ALG biocomposite was characterized using several complementary analytical methods that include CHN-O, XRD, FTIR, SEM-EDX, and pHpzc. This new adsorbent, named CHT-GLX/ALG, was utilized for the adsorption of a cationic dye (methyl violet 2B; MV 2B), from synthetic wastewater. The optimization of the dye adsorption process involved key parameters is listed: CHT-GLX/ALG dosage (from 0.02 to 0.1 g/100 mL), pH (from 4 to 10), and contact time (from 20 to 180 min) that was conducted using the Box-Behnken design (BBD). The optimal adsorption conditions for the highest decolorization efficiency of MV 2B (97.02 %) were estimated using the statistical model of the Box-Behnken design. These conditions include a fixed adsorbent dosage of 0.099 g/100 mL, pH 9.9, and a 179.9 min contact time. The empirical data of MV 2B adsorption by CHT-GLX/ALG exhibited favorable agreement with the Freundlich isotherm model. The kinetic adsorption profile of MV 2B by CHT-GLX/ALG revealed a good fit with the pseudo-second-order model. The maximum adsorption capacity (qmax) for MV 2B by CHT-GLX/ALG was estimated at 110.8 mg/g. The adsorption of MV 2B onto the adsorbent can be attributed to several factors, including electrostatic interactions between the negatively charged surface of CHT-GLX/ALG and the MV 2B cation, as well as n-π and H-bonding. These interactions play a crucial role in facilitating the effective adsorption of MV 2B onto the biocomposite adsorbent. Generally, this study highlights the potential of CHT-GLX/ALG as an efficient and sustainable adsorbent for the effective removal of organic dyes.

Production of activated carbon from food wastes (chicken bones and rice waste) by microwave assisted ZnCl2 activation: an optimized process for crystal violet dye removal.

A major worldwide challenge that presents significant economic, environmental, and social concerns is the rising generation of food waste. The current work used chicken bones (CB) and rice (R) food waste as alternate precursors for the production of activated carbon (CBRAC) by microwave radiation-assisted ZnCl2 activation. The adsorption characteristics of CBRAC were investigated in depth by removing an organic dye (crystal violet, CV) from an aquatic environment. To establish ideal conditions from the significant adsorption factors (A: CBRAC dosage (0.02-0.12 g/100 mL); B: pH (4-10); and C: duration (30-420), a numerical desirability function of Box-Behnken design (BBD) was utilized. The highest CV decolorization by CBRAC was reported to be 90.06% when the following conditions were met: dose = 0.118 g/100 mL, pH = 9.0, and time = 408 min. Adsorption kinetics revealed that the pseudo-first order (PFO) model best matches the data, whereas the Langmuir model was characterized by equilibrium adsorption, where the adsorption capacity of CBRAC for CV dye was calculated to be 57.9 mg/g. CV adsorption is accomplished by several processes, including electrostatic forces, pore diffusion, π-π stacking, and H-bonding. This study demonstrates the use of CB and R as biomass precursors for the efficient creation of CBRAC and their use in wastewater treatment, resulting in a greener environment.

The novelty of this research work relates to converting food wastes (mixture of chicken bones and rice waste) into activated carbon via microwave assisted ZnCl₂ activation. Moreover, the produced activated carbon was successfully applied as a potential adsorbent for removal of a toxic cationic dye; namely, crystal violet (CV) from aqueous environment.

Fabrication of Fe3O4 core-TiO2/mesoSiO2 and Fe3O4 core-mesoSiO2/TiO2 Double Shell Nanoparticles for Methylene Blue Adsorption: Kinetic, Isotherms and Thermodynamic Characterization.

Herein, Fe3O4 core-TiO2/mesoSiO2 and Fe3O4 core-mesoSiO2/TiO2 double shell nanoparticles were prepared by first (R1) and second (R2) routes and applied for the removal of methylene blue. The reported adsorption capacities for R1-0.2, R1-0.4 and R2 samples were 128, 118 and 133 mg.g-1, respectively, which were obtained after 80 min as equilibrium contact time, and pH of 6 using a methylene blue concentration of 200 ppm. The adsorption of methylene blue using the prepared Fe3O4 core-meso SiO2/TiO2 double shell was analyzed by kinetic and isotherms models. In addition, thermodynamic investigations were applied to assess the spontaneous nature of the process. The obtained results confirmed that the pseudo-second order model is well fitted with the adsorption data and the Freundlich-isotherm assumption suggested a multilayer adsorption mechanism. In addition, results of the thermodynamic investigation indicated that ΔG° was in the range of -2.3 to -6.8 kJ/mol for R1-0.2, -2.8 to -6.3 kJ/mol for R1-0.4 and -2.0 to -5.2 kJ/mol for R2. In addition, the ΔH° and ΔS° values were found in the range of 26.4 to 36.19 kJ.mol-1 and 94.9 to 126.3 Jmol-1 K-1, respectively. These results confirm that the surfaces of Fe3O4 core-mesoSiO2/TiO2 and Fe3O4 core-TiO2/mesoSiO2 double shell exhibit a spontaneous tendency to adsorb methylene blue from the aqueous solutions. The achieved performance of Fe3O4 core-meso SiO2/TiO2 and Fe3O4 core-TiO2/meso SiO2 double shell as adsorbent for methylene blue removal will encourage future research investigations on the removal of a broad range of contaminants from wastewater.

A promising sensing platform for explosive markers: Zeolite-like metal-organic framework based monolithic composite as a case study.

Preconcentration for on-site detection or subsequent determination is a promising technique for selective sensing explosive markers at low concentrations. Here, we report divinylbenzene monolithic polymer in its blank form (neat-DVB) and as a composite incorporated with sodalite topology zeolite-like metal-organic frameworks (3-ZMOF@DVB), as a sensitive, selective, and cost-effective porous preconcentrator for aliphatic nitroalkanes in the vapor phase as explosive markers at infinite dilution. The developed materials were fabricated as 18 cm gas chromatography (GC) monolithic capillary columns to study their separation performance of nitroalkane mixture and the subsequent physicochemical study of adsorption using the inverse gas chromatography (IGC) technique. A strong preconcentration effect was indicated by a specific retention volume adsorption/desorption ratio equal to 3 for nitromethane on the neat-DVB monolith host-guest interaction, and a 14% higher ratio was observed using the 3-ZMOF@DVB monolithic composite despite the low percentage of 0.7 wt.% of sod-ZMOF added. Furthermore, Incorporating ZMOF resulted in a higher percentage of micropores, increasing the degree of freedom more than bringing stronger adsorption and entropic-driven interaction more than enthalpic. The specific free energy of adsorption (ΔGS) values increased for polar probes and nitroalkanes, denoting that adding ZMOFs earned the DVB monolithic matrix a more specific character. Afterward, Lewis acid-base properties were calculated, estimating the electron acceptor (KA) and electron donor (KB) constants. The neat-DVB was found to have a Lewis basic character with KB/KA = 7.71, and the 3-ZMOF@DVB had a less Lewis basic character with KB/KA = 3.82. An increased electron-accepting nature can be directly related to incorporating sod-ZMOF into the DVB monolithic matrix. This work considers the initial step in presenting a portable explosives detector or preconcentrating explosive markers trace prior to more sophisticated analysis. Additionally, the IGC technique allows for understanding the factors that led to the superior adsorption of nitroalkanes for the developed materials.